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SRX14526709: UCE sequences of Nixonia_watshami_USNMENT01109338
1 ILLUMINA (Illumina HiSeq 2500) run: 3.9M spots, 983.2M bases, 499.4Mb downloads

Design: Samples were extracted destructively or non-destructively using Qiagen DNeasy extraction kits. DNA was sheared to 400-600 bp using a QSonica Q800R sonicator. Genomic libraries were prepared using Kapa Hyper Prep Kits (1/4 volume reactions) and iTru, 8bp dual-indexing adapters (Glenn et al. 2019). Samples were pooled into pools of 8-10 samples and enriched for ultraconserved elements (UCEs) using custom-designed probes (MYcroarray, Inc., now ArborBiosciences, Ann Arbor, MI) targeting 2590 UCE loci in Hymenoptera (myBaits UCE Hymenoptera 2.5Kv2P, Branstetter et al. (2017)). Enriched pools were pooled together (up to 100 individual samples) and shipped to the University of Utah genomics core facility for sequencing on an Illumina HiSeq 2500 instrument (2x125 bp v4 chemistry). Submitted here are raw, untrimmed sequencing reads. For trimming raw reads, use i7:GATCGGAAGAGCACACGTCTGAACTCCAGTCAC*ATCTCGTATGCCGTCTTCTGCTTG and i5:AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT*GTGTAGATCTCGGTGGTCGCCGTATCATT, where * indicates the index sequence. Adapter (index) sequences are specified as "i7-iTru-i7_i5-iTru-i5 in the sequence file names.
Submitted by: Museum fuer Naturkunde
Study: Key innovations and the diversification of Hymenoptera
show Abstracthide Abstract
The order Hymenoptera (wasps, ants, sawflies, and bees) represents one of the most diverse animal lineages, but whether specific key innovations have contributed to its diversification is still unknown. We assembled the largest time-calibrated phylogeny of Hymenoptera to date and investigated the origin and possible correlation of particular morphological and behavioral innovations with diversification in the order: the wasp waist of Apocrita; the stinger of Aculeata; parasitoidism, a specialized form of carnivory; and secondary phytophagy, a reversal to plant-feeding. Here, we show that parasitoidism has been the dominant strategy since the Late Triassic in Hymenoptera, but was not an immediate driver of diversification. Instead, transitions to secondary phytophagy (from parasitoidism) had a major influence on diversification rate in Hymenoptera. Support for the stinger and the wasp waist as key innovations remains equivocal, but these traits may have laid the anatomical and behavioral foundations for adaptations more directly associated with diversification.
Sample:
SAMN26355913 • SRS12324455 • All experiments • All runs
Library:
Name: Nixonia_watshami_USNMENT01109338
Instrument: Illumina HiSeq 2500
Strategy: Targeted-Capture
Source: GENOMIC
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 3.9M spots, 983.2M bases, 499.4Mb
Run# of Spots# of BasesSizePublished
SRR183926143,932,851983.2M499.4Mb2023-02-06

ID:
20726924

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